TABLE 1.
Target virulence genes and antimicrobial resistance genes, primer sequences, amplicon sizes and annealing temperatures.
| Target gene | Primer sequence (5’ – 3’) | Product size (bp) | Annealing temperature (°C) | References |
|---|---|---|---|---|
| asp | F-GGTATGATTTCTACAAAGCGAGA R-ATAAAAGACTATCGTCGCGTG |
500 | 53 | Al Amri et al. (2007) |
| hipO | F-GAAGAGGGTTTGGGTGGT R-AGCTAGCTTCGCATAATAACTTG |
735 | 53 | Al Amri et al. (2007) |
| gyrA | F-GAAGAATTTTATATGCTATG R-TCAGTATAAC GCATCGCAGC |
235 | 53 | Chatur et al. (2014) |
| gyrA | F-ACGCAAGAGAGATGGTT R-TCAGTATAACGCATCGCAGC |
270 | 45 | Chatur et al. (2014) |
| blaOXA-61 | F- AGAGTATAATACAAGCG R- TAGTGAGTTGTCAAGCC |
372 | 54 | Obeng et al. (2012) |
| tetO | F-GGCGTTTTGTTTATGTGCG R-ATGGACAACCCGACAGAAGC |
559 | 49 | Gibreel et al. (2004) |