Figure 5. Neuronal cell death is influenced by the CL-remodeling pathway after mechanical stretch injury.

(A) Quantification of cytotoxicity by CCK8 at 48 hours after transient transfection with iPLA₂γ plasmid (P) and at 24 hours after stretch (S). Transfection efficiency was measured by Western blot analysis at 48 hours. n = 6/group, *P < 0.05 vs. vector control (VC), ^#P < 0.05 vs. vector plus S (vector + S), 1-way ANOVA. (B) Cell death measured by Annexin V-APC/7-AAD at 48 hours after transient transfection with P and at 24 hours after stretch. n = 3/group, *P < 0.05 vs. VC; ^#P < 0.05 vs. S by 1-way ANOVA. P, iPLA₂γ overexpression group. S, Stretch group: P+S, iPLA2(gamma) overexpression + stretch group (C) Quantification of cytotoxicity in Taz siRNA KD cells after stretch injury. n = 4/group, *P < 0.05 vs. scrambled siRNA control (SC), 1-way ANOVA. (D) Effect of Taz overexpression (OE) on mechanical stretch–induced cytotoxicity. n = 3/group, *P < 0.05 vs. VC, 1-way ANOVA. (E) Mitochondrial membrane potential measured by JC-1 in Taz-overexpressing and empty vector transfection cells after stretch injury. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) (10 μM) was used as a positive control. n = 4/group, *P < 0.05 vs. VC, 1-way ANOVA.