TABLE 1.
Target virulence genes, primer sequences, amplicon sizes and annealing temperatures.
| Target gene | Primer sequence (5’–3’) | Product size (bp) | Annealing temperature (°C) | References |
|---|---|---|---|---|
| cadF | F-TTGAAGGTAATTTAGATATG R-CTAATACCTAAAGTTGAAAC |
400 | 43 | Chansiripornchai and Sasipreeyajan (2009) |
| asp | F-GGTATGATTTCTACAAAGCGAGA R-ATAAAAGACTATCGTCGCGTG |
500 | 53 | Al Amri et al. (2007) |
| hipO | F-GAAGAGGGTTTGGGTGGT R-AGCTAGCTTCGCATAATAACTTG |
735 | 53 | Al Amri et al. (2007) |
| ciaB | F-TGCGAGATTTTTCGAGAATG R-TGCCCGCCTTAGAACTTACA |
527 | 54 | Chansiripornchai and Sasipreeyajan (2009) |
| dnaJ | F-ATTGATTTTGCTGCGGGTAG R-ATCCGCAAAAGCTTCAAAAA |
177 | 50 | Chansiripornchai and Sasipreeyajan (2009) |
| pldA | F-AAGAGTGAGGCGAAATTCCA R-GCAAGATGGCAGGATTATCA |
385 | 46 | Chansiripornchai and Sasipreeyajan (2009) |
| cdtA | F-CCTTGTGATGCAAGCAATC R-ACACTCCATTTGCTTTCTG |
370 | 49 | Rizal et al. (2010) |
| cdtB | F-GTTAAAATCCCCTGCTATCAACCA R-GTTGGCACTTGGAATTTGCAAGGC |
495 | 51 | Rizal et al. (2010) |
| cdtC | F-CGATGAGTTAAAACAAAAAGATA R-TTGGCATTATAGAAAATACAGTT |
182 | 48 | Rizal et al. (2010) |
Note: Please see the full reference list of the article, Reddy, S. & Zishiri, O.T., 2018, ‘Genetic characterisation of virulence genes associated with adherence, invasion and cytotoxicity in Campylobacter spp. isolated from commercial chickens and human clinical cases’, Onderstepoort Journal of Veterinary Research 85(1), a1507. https://doi.org/10.4102/ojvr.v85i1.1507, for more information.