Fig. 4.

Sensor cross talk on the multiplexed assay based on the bio-interface. a. Assay can be performed directly on a functionalized 1 μm thick glass grown on the chip surface. b,c. Electromagnetic simulation of the fluorescence emission from a 50 μm diameter spot on the surface and its coupling in the lateral dimensions. Since the pixels are 100 μm in dimension, the emission remains localized to the pixel underneath the spot minimizing cross-talk. d. To re-use chip to do multiple measurements in a laboratory setting, we create a disposable bio-interface that is aligned with the sensor array with silicon positioners (Fig. 1). We perform the experiments on the glass slip positioned about 100 μm from the chip surface. e,f. Electromagnetic simulation of the fluorescence emission from a 50 μm diameter spot on the glass slip. The elevation causes the emission to spread creating cross-talk among the neighboring pixels. This limits the number of spots we can perform on the surface and is only done to reuse the chip for multiple experiments in a laboratory setting. The functionalization can be done on the chip surface as shown in part a., similar to [33].