Fig. 1.

Monocyte isolation procedure and flow cytometer measurement. (a) The three-step isolation procedure of monocytes from whole blood is illustrated. At the first step a gradient separation with ‘Ficoll’ was performed. The obtained PBMC ring is taken and suspended in PBS. Magnetic Ab-beads are added in the second step. Afterwards in the third step a magnet retains the bound content to the falcon tube surface, while monocytes remain free in the suspension to analyze. (b) Flow cytometer control of isolated monocytes (red) and added lymphocytes (gray), where two distinct zones are noticed. Monocytes show significant bigger forward and side scattering intensity values compared to lymphocytes.