Fig. 2.
Monocyte observations. (a) Bright-field observation with 100× oil immersion objective of monocytes in 0.2g dL−1 PEO-PBS. Trypan blue investigations with a 20x objective in color before (b) and after (c) cell measurements. (d) 3D reconstruction of confocal fluorescent z-stack images (ImageJ: plugin/Stacks - Z-functions/360- 3D project... Macro). (e) Confocal image of one slide of the fluorescent z-stack. (Green color shows the ‘Wheat germ Agglutinin’, while red color indicates the ‘Sytox Green’)