Skip to main content
. Author manuscript; available in PMC: 2018 Nov 16.
Published in final edited form as: Nature. 2018 May 16;557(7706):580–584. doi: 10.1038/s41586-018-0125-z

Extended Data Fig. 5 |. Absence of Tet2 expression in haematopoietic cells leads to barrier dysfunction and PMP.

Extended Data Fig. 5 |

a, Intestinal permeability measurement by blood plasma FITC-dextran concentrations (n = 16 (Tet2f/fcre−), 6 (Tet2f/fVavcre), 12 (Tet2f/fVillincre) or 12 (Tet2f/fLysMcre) mice). b, c, Gene expression of antimicrobial peptides Retlnb (b left), Ang4 (b right) and (c) tight junction genes ZO-1 (Tjp1) (left), occludin (Ocln) (middle), and desmoplakin (Dsp) (right) in the jejunum (n = 13 (Tet2f/fcre−), 7 (Tet2f/fVavcre), 9 (Tet2f/fVillincre) or 10 (Tet2f/fLysMcre) mice). d, Representative dot blots (left) and numbers of LSK cells (right) in the spleen, CD11b+Gr1+ myeloid cells in the spleen (n = 6 (Tet2f/fcre−), 12 (Tet2f/fVavcre), 10 (Tet2f/fVillincre) or 10 (Tet2f/fLysMcre) mice) and peripheral blood, and lymphocytes in the peripheral blood (n = 10 (Tet2f/fcre−) 8 (Tet2f/fVavcre), 10 (Tet2f/fVillincre) or 10 (Tet2f/fLysMcre) mice). e, White blood cell (WBC) count (top) and cell number per ml of blood of CD11b+ monocytes (bottom) (n = 10 (Tet2f/fcre−) 8 (Tet2f/fVavcre), 10 (Tet2f/fVillincre) or 10(Tet2f/fLysMcre) mice). f, Spleen weight is shown (n = 10 (Tet2f/fcre−) 6 (Tet2f/fVavcre), 10 (Tet2f/fVillincre) or 10 (Tet2f/fLysMcre) mice). In a–f, centre is mean, one-way ANOVA, Sidak’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data are representative of three independent experiments.