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. 2018 Oct 30;9(85):35581–35597. doi: 10.18632/oncotarget.26272

Figure 1. Effect of nasally administered MSC on cisplatin-induced cognitive impairments.

Figure 1

Male mice were treated with cisplatin (2.3 mg/kg/day) for two 5-day cycles with 5 days of rest in between, followed by 2 doses of 1x106 MSC administered nasally at 48- and 96-h post-cisplatin. (A) The PBT was performed 7-10 days after the last dose of MSC. Each animal was given 3 consecutive trials per day for the first 3 days and 2 consecutive trials on day 4. The test is divided into 3 difficulties, easy (trials 1-4), intermediate (trials 5-7), and difficult (trials 8-11). During the easy trials, the animals are allowed free passage between the light and dark compartment via the connecting tunnel. In the intermediate trials, the tunnel is covered with normal bedding and animals must burrow through to reach the dark side. In the difficult trials, the tunnel is blocked by a lid and the animals must learn to remove the blockage before they can escape to the dark compartment. Time until entrance into the dark compartment is recorded. Results are expressed as mean ± SEM; n = 8 in 2 separate cohorts. Tukey post hoc: *P < 0.05 versus PBS controls. (B) NOPRT was performed 4 days after the completion of the PBT. The discrimination index was calculated as (TNovel – TFamiliar)/(TNovel + TFamiliar); 0 represents no preference for the novel object. Results are expressed as means ± SEM; n = 7-8 in 2 separate cohorts. Two-way ANOVA Cisplatin x MSC interaction (F [1, 26] = 12.98, p = 0.0013), Tukey post hoc: *P < 0.05 versus PBS controls. (C) Total interaction times in the NOPRT were not affected by cisplatin and MSC treatment. Two-way ANOVA Cisplatin x MSC interaction (F [1, 26] = 0.0792, p = 0.7805). (D) The percentage spontaneous alternation in a Y-maze was determined 1 day after the completion of the NOPRT. Dotted line indicates random chance. Results are expressed as means ± SEM; n = 7-8. Two-way ANOVA Cisplatin x MSC interaction (F [1, 26] = 4.786, p = 0.0379), Tukey post hoc: *P < 0.05. (E) Total arm entries in the Y-maze were not affected by cisplatin and MSC treatment. n = 7-8. Two-way ANOVA Cisplatin x MSC interaction (F [1, 26] = 0.0135, p = 0.9083).