Figure 6. Effects of nasally administered MSC on cisplatin-induced changes in mitochondrial respiratory function and mitochondrial morphology.

(A) Mice were treated with cisplatin for two 5-day cycles followed by 2 doses of 1×10⁶ MSC nasally at 48 h and 96 h post cisplatin. Synaptosomes were isolated 3 weeks after the last dose of MSC. Results are expressed as means ± SEM; n = 10. Two-way ANOVA, Tukey post hoc: ^*P < 0.05 versus PBS controls. Basal, basal respiration; Max, maximal respiratory capacity; SRC, Spare respiratory capacity. (B) At 48 h after completion of cisplatin treatment, synaptosomes were isolated from the brains of animals treated with cisplatin for two 5-day cycles. Oxygen consumption rates were analyzed using the Seahorse XFe 24 Analyzer. Basal, max, and SRC were calculated. Results are expressed as means ± SEM; n = 5. T-test: ^*P < 0.05. Basal: p = 0.6394; Max: p = 0.0116; SRC: p = 0.0071. (C) Synaptosomes were isolated from the brains of mice treated with PBS, cisplatin, MSC, or cisplatin + MSC at 3 weeks after the last dose of MSC. (D) Mitochondrial width and (E) and percentage of atypical mitochondria were quantified. Results are expressed as means ± SEM; n = 4. Two-way ANOVA, Tukey post hoc: ^*P < 0.05 versus PBS controls.