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. 2018 Nov 13;11:8033–8044. doi: 10.2147/OTT.S174637

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© 2018 Mu et al. This work is published and licensed by Dove Medical Press Limited

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Establishment of HOTTIP-silenced OTSCC cell strains.

Notes: (A) Expression levels of HOTTIP were detected by real-time PCR in human tongue squamous carcinoma cell lines. (B and C) TSCCA and TCA8113 cells were transfected with HOTTIP shRNA or NC shRNA, and then selected for stable expression. The levels of HOTTIP mRNA in cells were examined by real-time PCR. GAPDH served as the internal control. Compared with NC shRNA, **P-value <0.01.

Abbreviations: HOTTIP, HOXA transcript at the distal tip; OTSCC, oral tongue squamous cell carcinoma; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NC, negative control.