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. 2018 Nov 13;11:8033–8044. doi: 10.2147/OTT.S174637

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© 2018 Mu et al. This work is published and licensed by Dove Medical Press Limited

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Silencing of HOTTIP inhibited proliferation and arrested cell cycle of OTSCC cells.

Notes: (A and B) The proliferation of HOTTIP-silenced TSCCA and TCA8113 cells was assessed by the CCK-8 assay. (C and D) Doubling time was determined by cell counting. (E and F) Colony formation rate was detected by the 2D clonogenic assay. (G and H) Cell cycle was analyzed by flow cytometry. (I and J) The protein expression levels of cyclin B, D1, and E were determined by Western blot analysis. *P<0.05 and **P<0.01 compared with NC shRNA.

Abbreviations: HOTTIP, HOXA transcript at the distal tip; OTSCC, oral tongue squamous cell carcinoma; CCK-8, Cell Counting Kit-8; NC, negative control; h, hours.

Silencing of HOTTIP inhibited proliferation and arrested cell cycle of OTSCC cells.

Notes: (A and B) The proliferation of HOTTIP-silenced TSCCA and TCA8113 cells was assessed by the CCK-8 assay. (C and D) Doubling time was determined by cell counting. (E and F) Colony formation rate was detected by the 2D clonogenic assay. (G and H) Cell cycle was analyzed by flow cytometry. (I and J) The protein expression levels of cyclin B, D1, and E were determined by Western blot analysis. *P<0.05 and **P<0.01 compared with NC shRNA.

Abbreviations: HOTTIP, HOXA transcript at the distal tip; OTSCC, oral tongue squamous cell carcinoma; CCK-8, Cell Counting Kit-8; NC, negative control; h, hours.