Figure 3.

Knockdown of HOTTIP induced apoptosis of OTSCC cells.

Notes: (A and B) Apoptosis of TSCCA and TCA8113 cells was examined by flow cytometry. (C and D) Cells were incubated with Hoechst stains, and the images of apoptotic cells were taken under a fluorescent microscope (scale bar =50 µm). (E and F) The expression levels of apoptosis markers were detected by Western blot analysis with GAPDH as the internal control. *P<0.05 and **P<0.01 compared with NC shRNA.

Abbreviations: HOTTIP, HOXA transcript at the distal tip; OTSCC, oral tongue squamous cell carcinoma; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NC, negative control.