Figure 4.

HOTTIP silencing suppressed the migration and invasion of OTSCC cells.

Notes: (A and B) Scratch wound healing assay and Matrigel-based transwell assay were used to evaluate the migration and invasion of TSCCA and TCA8113 cells (scale bar=100 µm). (C and D) Immunofluorescence assay using E-cadherin antibody (red) was performed to detect the expression of E-cadherin in OTSCC cells. DAPI was used to visualize the cell nucleus (blue; scale bar=50 µm). (E and F) E-cadherin protein and mRNA levels were determined by Western blotting and real-time PCR, respectively. *P<0.05 and **P<0.01 compared with NC shRNA.

Abbreviations: HOTTIP, HOXA transcript at the distal tip; OTSCC, oral tongue squamous cell carcinoma; NC, negative control.