Figure 5.

Inhibition of HOTTIP delayed tumor formation in vivo.

Notes: (A and B) Parental and transfected OTSCC cells with HOTTIP shRNA or NC shRNA were inoculated subcutaneously into nude mice, and xenograft volumes were measured every 3 days. (C and D) By the end of week 3, the mice were sacrificed, and the xenograft tumors were excised and weighted. (E and F) H&E staining was used to investigate the histological change in tumor tissues (scale bar =100 µm). (G and H) The mRNA levels of HOTTIP in the tumors were analyzed by real-time PCR. **P<0.01 compared with NC shRNA.

Abbreviations: HOTTIP, HOXA transcript at the distal tip; OTSCC, oral tongue squamous cell carcinoma; NC, negative control.