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. 2018 Dec;24(12):1738–1748. doi: 10.1261/rna.067090.118

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© 2018 He et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 1. — Dynamic expression pattern of zebrafish slbp2 during oogenesis and embryogenesis. (A) slbp2 and slbp1 expression in adult tissue detected by RT-PCR and Slbp2 protein detected by western blot. (B) Slbp2 expression in oocytes during oogenesis detected by western blot. (C) slbp2 and slbp1 expression in embryos during embryogenes is detected by RT-PCR and Slbp2 protein detected by western blot. (D) slbp2 mRNA localization during oogenesis. Ovarian cross sections after in situ hybridization with antisense (D1) or sense (D2) slbp2 probe. (E) Slbp2 protein localization in zebrafish mature ovary. Ovarian cross-section was stained with DAPI and anti-Slbp2 antibody (E1) or preimmune serum (E2). I, primary growth stage; II, cortical alveolus stage; III, vitellogenic stage; IV, maturing oocyte stage; V, mature eggs.