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. 2018 Dec;24(12):1738–1748. doi: 10.1261/rna.067090.118

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© 2018 He et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 5. — Zebrafish Slbp2 regulates the storage of histone mRNAs and proteins during zebrafish oogenesis. (A) Relative expression by qPCR detection of four replication-dependent histones in WT and Zslbp2Δ4^−/− ovaries, or WT and MZslbp2Δ4^−/− embryos at the two-cell, 256-cell, and high stages. ef1α was used as the control. Each bar represents mean ± SD (n = 3). Asterisks (*) indicate significant differences (P < 0.05) between WT and Zslbp2Δ4^−/− ovaries, or WT and MZslbp2Δ4^−/− embryos. Data were performed from three independent experiments. (B) Western blot detection of H2b and H3 proteins in WT and Zslbp2Δ4^−/− ovaries, and WT and MZslbp2Δ4^−/− embryos at two-cell stage and high stage. The developmental stages of embryos are marked at the bottom. (C) Relative expression by qPCR detection of three histone variants in WT and MZslbp2Δ4^−/− embryos at the 256-cell stage and high stage. ef1α was used as the control. Each bar represents mean ± SD (n = 3). Asterisks (*) indicate significant differences (P < 0.05) between WT and MZslbp2Δ4^−/− embryos. (D) EMSA analysis of the binding ability between Slbp2 and SL of H2a mRNA.