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. 2018 Dec;24(12):1647–1658. doi: 10.1261/rna.066290.118

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© 2018 Paggi and Bejerano; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 4. — Branchpoints display a nucleotide content signature, consistent with extended base-pairing with U2 snRNA. This signature is stronger in branchpoints lacking a −2 U (B) than in branchpoints with a −2 U (A). (C) C branchpoints display a strong increase in C nucleotide content in this same position. (D) The human and yeast U2 snRNA sequence positioned for interacting with this upstream recognition element (top). P represents psuedouridines and (*) corresponds to the branchpoint position. The canonical branchpoint motif (upper case) and positions of G, C content signature (lower case). (E) An image from a cryo-EM structure of the spliceosome (PDB ID: 5O9Z) shows an extended intron-U2 snRNA duplex. The intronic sequence is shown in gray, except for the branchpoint which is shown in red. The U2 snRNA sequence is colored by base (red, blue, green, and magenta for A, C, G, and T, respectively).