Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec;24(12):1667–1676. doi: 10.1261/rna.067660.118

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Dao et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

FIGURE 4. — Temperature dependence of C1397 conformational dynamics. (A) In the decoding region of the ambient-temperature mRNA model, C1397 engages in H-bonding with U4 which demarcates the boundary between the A-site mRNA codon and codon-1. A final unbiased F_o-F_c simple omit ambient temperature electron-density map of mRNA and C1397 contoured at the 3σ level is shown in blue and at 3 Å. (B) Superposition of the ambient temperature 30S-decoding complex with cryogenic structure shows disorder in the cryogenic mRNA structure as well as the alternate conformation of C1397. (C) Superposition of our ambient temperature structure (salmon) and the identical cryo temperature structure obtained by another laboratory (slate; PDB ID: 1IBL) showing the disorder in the cryogenic structure. (D) Superposition of our ambient temperature structure (salmon) with the two cryo-cooled structures (cyan and slate).