Figure 3.
Translocation of Rhod6G Solution or Rhod6G–CAP–NPs to Vaginal Epithelial cells (VK2/E6E7). Rhod6G Solution (red) or Rhod6G–CAP–NPs at: 30 min (A–C); and seven days (D–F) post-treatment. Orthogonal Plane Images were obtained and evaluated by the IBIF Leica TCS SP8 MP Confocal Microscope at 63× with 5× computational magnification. DAPI: blue, DiO: green, Rhodamine: red. (A)/(D): Untreated, (B)/(E): Rhodamine Solution (1 μg/mL), (C)/(F): Rhod6G–CAP–NPs (1 μg/mL). Scale bar = 13 μm and is applicable to (B–F). For visualization of nanoparticles in print, scale bars are omitted from (B–F), and contrast and brightness were enhanced using imaging software.