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. 2017 Sep 7;9(9):423. doi: 10.3390/polym9090423

Figure 3.

Figure 3

Translocation of Rhod6G Solution or Rhod6G–CAP–NPs to Vaginal Epithelial cells (VK2/E6E7). Rhod6G Solution (red) or Rhod6G–CAP–NPs at: 30 min (AC); and seven days (DF) post-treatment. Orthogonal Plane Images were obtained and evaluated by the IBIF Leica TCS SP8 MP Confocal Microscope at 63× with 5× computational magnification. DAPI: blue, DiO: green, Rhodamine: red. (A)/(D): Untreated, (B)/(E): Rhodamine Solution (1 μg/mL), (C)/(F): Rhod6G–CAP–NPs (1 μg/mL). Scale bar = 13 μm and is applicable to (BF). For visualization of nanoparticles in print, scale bars are omitted from (BF), and contrast and brightness were enhanced using imaging software.