. Author manuscript; available in PMC: 2018 Nov 16.

Published in final edited form as: Toxicol Mech Methods. 2017 Jul 20;27(9):707–716. doi: 10.1080/15376516.2017.1351019

Table 4.

Consistency^a of actual mixture (MX) vs. predicted dose-addition (DA) and independence (I) concentrations (mg/L) and combined effects values for the mixtures

		EC_25+50+70^b
Time	Experiment #	MX	DA	I	MX/DA^c	MX/I^d	AQ₅₀^e	IQ₅₀^f
15-min	MX – 1	46.95	49.50	40.52	0.95	1.16	0.91	1.05
	MX – 2	44.37	44.57	35.66	1.00	1.24	0.97	1.15
	MX – 3	40.99	42.92	34.40	0.96	1.19	0.92	1.09
	MX – 4	42.82	45.87	35.70	0.93	1.20	0.91	1.09
	MX – 5	36.80	39.42	30.90	0.93	1.19	0.90	1.07
	MX – 6	35.62	36.85	29.27	0.97	1.22	0.90	1.08
	mean	41.26^g	43.19^g	34.41^h	0.96ⁱ	1.20^j	0.92^k	1.09^l
	CV	*10.6*	*10.5*	*11.6*	*2.5*	*2.4*	*3.0*	*3.1*
30-min	MX – 1	35.35	34.38	30.22	1.03	1.17	1.01	1.09
	MX – 2	33.03	32.32	27.82	1.02	1.19	1.00	1.11
	MX – 3	31.42	31.91	27.28	0.98	1.15	0.96	1.08
	MX – 4	32.90	34.57	28.70	0.95	1.15	0.93	1.08
	MX – 5	27.97	30.17	26.09	0.93	1.07	0.89	0.98
	MX – 6	27.17	27.47	23.27	0.99	1.17	0.96	1.09
	mean	31.31^m	31.80^m	27.23ⁿ	0.98ⁱ	1.15^j	0.96^k	1.07^l
	CV	*10.1*	*8.4*	*8.8*	*4.0*	*3.5*	*4.8*	*4.4*
45-min	MX – 1	29.68	27.88	25.26	1.06	1.18	1.06	1.12
	MX – 2	27.85	26.63	23.85	1.05	1.17	1.05	1.11
	MX – 3	26.30	26.43	23.24	1.00	1.13	0.97	1.09
	MX – 4	28.37	29.47	25.81	0.96	1.10	0.94	1.05
	MX – 5	23.61	25.25	22.29	0.94	1.06	0.90	1.00
	MX – 6	22.07	23.22	20.99	0.95	1.05	0.93	0.98
	mean	26.32^o	26.48^o	23.57^o	0.99ⁱ	1.11^j	0.97^k	1.06^l
	CV	*11.1*	*8.1*	*7.7*	*5.3*	*4.7*	*5.8*	*5.5*

mean and coefficient of variation (CV) values obtained from data to four decimal places

sum of the EC₂₅, EC₅₀, and EC₇₅ values expressed as 3C2B-equivalent concentrations

MX/DA = MX EC_25+50+75 / DA EC_25+50+75 at a given exposure time

MX/I = MX EC_25+50+75 /I EC_25+50+75 at a given exposure time

dose-additivity quotient value at the EC₅₀ only

independence quotient value at the EC₅₀ only

^g,h

grouped means with different letters were statistically different (Holm-Sidak test, p < 0.05)

^i,j

mean MX/DA and MX/I values at that exposure time were statistically different (t-test, p ≤ 0.003)

^k,l

mean AQ₅₀ and IQ₅₀ values at that exposure time were statistically different (t-test, p ≤ 0.02 or lower)

^m,n

grouped EC_25+50+75 means with different letters were statistically different (Holm-Sidak test, p < 0.05)

grouped EC_25+50+75 means were not significantly different in one-way ANOVA