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Myotubes differentiated from non-diseased hiPSC-derived myogenic progenitors, primary human skeletal myoblasts, mESC-derived myogenic progenitors, and C2C12 cells could also align nearly perpendicular to nanogrooves on Matrigel-functionalized substrates, suggesting that this phenotype might be universal for muscle cells regardless of their origins and species. Images are shown as in Figure 2a. The cells were cultured for 2 weeks after differentiation induction.
