KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| NFAT1 in 1:200 (IF) or 1:1000(WB), Rabbit | Cell Signaling Technology | Cat#5861S, RRID:AB_10835147 |
| pS6K(T389) in 1:1000(WB), Rabbit | Cell Signaling Technology | Cat#9234S, RRID:AB_2269803 |
| pCamKII(T286) in 1:1000 (WB), Rabbit | Cell Signaling Technology | Cat#12716S, RRID:AB_2713889 |
| MeCP2 in 1:200 (IF) or 1:1000(WB), Rabbit | Cell Signaling Technology | Cat#3456S, RRID:AB_2143849 |
| pMeCP2(S421) in 1:1000(WB), Rabbit | Rockland | Cat#600-401-X14, RRID:AB_2614557 |
| Firefly luciferase in 1:5000 (WB), Rabbit | Thermo | Cat#PA5-32209, RRID:AB_2549682 |
| SP1 antibody (Chip grade) | Cell Signaling Technology | CST # 9389S, RRID:AB_11220235 |
| normal rabbit IgG | Cell Signaling Technology | CST # 2729S, RRID:AB_1031062 |
| Cx43 in 1:200 (IF), Rabbit | Sigma | Cat#C6219, RRID:AB_476857 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| D-luciferin | Goldbio | Cat# LUCNA-1G |
| Carbenoxolone | Sigma | Cat# C4790 |
| Mefloquine | Sigma | Cat#1379059 |
| Danusertib | ApexBio | Cat# A4116 |
| Nifedipine | ApexBio | Cat#B1988 |
| SKF96365 | ApexBio | Cat#B6616 |
| Arsenic trioxide | Sigma | Cat# A1010 |
| John Hopkins University Clinical Compound Library | gift from Dr. Stephen T. C. Wong’s laboratory at Houston Methodist Research Institute | NA |
| Everolimus | ApexBio | Cat# A8169 |
| Critical Commercial Assays | ||
| Nextera XT DNA Library Prep Kit | Illumina | Cat# FC-131-1096 |
| Nextseq 500/550 high output v2 kit | Illumina | Cat# FC-404-2002 |
| Deposited Data | ||
| breast cancer metastases dataset | GEO | GSE46141 |
| human breast cancer metastases dataset in different organs | GEO | GSE14020 |
| Stroma cells in the breast cancer microenvironment | GEO | GSE27120 |
| Cancer-osteoblast heterotypic interactions dataset | GEO | GSE29036 |
| Subpopulations of MDA-MB-231 and Primary Breast Cancers | GEO | GSE2603 |
| Breast cancer relapse free survival and lung metastasis free survival | GEO | GSE5327 |
| Breast cancer relapse free survival | GEO | GSE2034 |
| Expression data from primary breast tumors | GEO | GSE12276 |
| Metastatic prostate cancer dataset | GEO | GSE77930 |
| in vivo Bone Lesion (IVBL) and Orthotopic tumors dataset | GEO | GSE110451 |
| ATO treatment on mesenchymal stem cells (MSCs) interacting breast cancer cells | GEO | GSE118923 |
| Experimental Models: Cell Lines | ||
| Human breast cancer MCF7 | ATCC | Cat# HTB-22, RRID:CVCL_0031 |
| Human breast cancer MDA-MB-361 | ATCC | Cat# HTB-27, RRID:CVCL_0620 |
| Mouse pre-osteoclast RAW264.7 | ATCC | Cat# TIB-71, RRID:CVCL_0493 |
| Human monocyte U937 | ATCC | Cat# CRL-1593.2, RRID:CVCL_0007 |
| Mouse breast cancer 4T1 | Barbara Ann Karmanos Cancer Center | RRID:CVCL_0125 |
| Mouse breast cancer 4TO7 | Barbara Ann Karmanos Cancer Center | RRID:CVCL_B383 |
| Mouse breast cancer 4T1.2 | Gift from Dr. Robin Anderson at Peter MacCallum Cancer Center | RRID:CVCL_GR32 |
| Human Mesenchymal stem cell | gift from Dr. Max Wicha’s laboratory at University of Michigan | |
| Human osteocyte MLO-Y4 | gift from Dr. Florent Elefteriou at Baylor College of Medicine | RRID:CVCL_M098 |
| Human Astrocyte | ScienceCell | Catalog #1800 |
| Human mammary fibroblast cell | gift from Dr. Eric Chang’s laboratory at Baylor College of Medicine | |
| Mouse fibroblast NIH3T3 | ATCC | Cat# CRL-1658, RRID:CVCL_0594 |
| Human pre-Osteoblast hFOB1.19 | ATCC | Cat#CRL-11372, RRID:CVCL_3708 |
| Human prostate cancer C4-2, Fluc+ | gift from Dr. Dasgupta Subhamoy’s lab at Roswell Park Comprehensive Cancer Center | |
| Human prostate cancer 22RV1 | ATCC | Cat#CRL-2505, RRID:CVCL_1045 |
| Experimental Models: Organisms/Strains | ||
| Mouse Bal/cJ | Envigo | NA |
| Mouse C57BL/6 | Envigo | NA |
| Athymic nude mice | Envigo | NA |
| Osx1-GFP::Cre mice | Jackson Laboratory | Cat#006361 |
| Gja1 flox mice | Jackson Laboratory | Cat#008039 |
| Cdh2 flox mice | Jackson Laboratory | Cat#007611 |
| Oligonucleotides | ||
| FAP-F | Tripathi et al., 2014 | TGGCGATGAACAATATCCTAGA |
| FAP-R | Tripathi et al., 2014 | ATCCGAACAACGGGATTCTT |
| ACTB-F | This paper | GTTGTCGACGACGAGCG |
| ACTB-R | This paper | GCACAGAGCCTCGCCTT |
| TWIST1_F | Tripathi et al., 2014 | AGCTACGCCTTCTCGGTCT |
| TWIST1_R | Tripathi et al., 2014 | TCCTTCTCTGGAAACAATGACA |
| MRC2_F | Tripathi et al., 2014 | AACTTCCGGGACAGTCTGG |
| MRC2_R | Tripathi et al., 2014 | TTACAGGGACTGTCGTTCCA |
| COL3AL_F | Tripathi et al., 2014 | ACTGGAGCACGGGGTCTT |
| COL3AL_R | Tripathi et al., 2014 | TCCTGGTTTCCCACTTTCAC |
| ANGPTL2_F | Tripathi et al., 2014 | GCCCACTATGCCCACTCTC |
| ANGPTL2_R | Tripathi et al., 2014 | CTGCAGGCAGTCTCTCCAT |
| MGP_F | Tripathi et al., 2014 | TGAAAGCATGGAATCTTATGAACTT |
| MGP_R | Tripathi et al., 2014 | CTGCTGAGGGGATATGAAGG |
| PTRF_F | Tripathi et al., 2014 | CAGCGTCAACGTGAAGACC |
| PTRF_R | Tripathi et al., 2014 | CCGGCAGCTTCACTTCAT |
| MECP2_F | This Paper | GCGAGGAGGAGAGACTGGA |
| MECP2_R | This Paper | TTTAAACTTGAGGGGTTTGTCC |
| GJA1-F | This Paper | TCCTCCTCTTTCTTGTTCAGTTTCTCT |
| GJA1-R | This Paper | CCTGCAGATCATATTTGTGTCTGTTC |
| GAPDH-F | This Paper | TTGAGGTCAATGAAGGGGTC |
| GAPDH-R | This Paper | GAAGGTGAAGGTCGGAGTCA |
| Actb-F | This Paper | GGCTGTATTCCCCTCCATCG |
| Actb-R | This Paper | CCAGTTGGTAACAATGCCATGT |
| Nfatc1_F | This Paper | TCCAAAGTCATTTTCGTGGA |
| Nfatc1_R | This Paper | CTTTGCTTCCATCTCCCAGA |
| Nfatc2_F | This Paper | CAACGGAAAGAGGAAACGAA |
| Nfatc2_R | This Paper | CTGTCTTGATGGCAGGGACT |
| Gapdh-F | This Paper | AGGTCGGTGTGAACGGATTTG |
| Gapdh-R | This Paper | TGTAGACCATGTAGTTGAGGTCA |
| FW-SP1-CX43 | This Paper | ACTGCTGCTCTTTGCCTCTT |
| RV-SP1-CX43 | This Paper | TGTAACTTGGAGCACAGAGCTT |
| Recombinant DNA | ||
| pMD2.G | Addgene | Cat#12259 |
| psPAX2 | Cat#12260 | |
| pwpt-Fluc/GFP or pwpt-Fluc/RFP | Wang et al., 2015 | |
| pHAGE-RSV-GCaMP6s | Addgene | Cat#80146 |
| pInducer22 | gift from Dr. Thomas Westbrook’s lab at Baylor College of Medicine | |
| pInducer22-DN-Ecad | Wang et al., 2015 | |
| pInducer22-WT-Cx43 | This paper | |
| pInducer22-DN-Cx43(G138R) | This paper | |
| pInducer22-DN-CaMKII(T286A) | This paper | |
| pInducer22-CA-CaMKII(T286D) | This paper | |
| pInducer22-DN-Calcineurin(delta H160Q) | This paper | |
| Software and Algorithms | ||
| ImageJ | National Institute of Health | NA |
| Cyverse | National Science Foundation | Award Numbers DBI-0735191 and DBI-1265383 |
| R 3.3.4 | R Core Team | NA |
| Graphpad Prism7 | GraphPad Software, Inc. | NA |
| Flow Jo v10.0. | FlowJo, LLC | NA |
| SPSS 19 | IBM | NA |
| Other | ||
| Code of analysis by R programming | This paper | https://github.com/lethesea/Targeting-calcium-signaling-in-bone-micrometastases |