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. 2018 Nov 14;38(6):BSR20180982. doi: 10.1042/BSR20180982

Figure 2. miR-199a-3p suppressed RA-FLS proliferation.

Figure 2

(A) RT-qPCR of miR-199a-3p in RA-FLSs transfected with pSilencer 4.1-miR-199a-3p plasmid (miR-199a-3p) or no-insert control pSilencer 4.1-CMV puro plasmid (Mock). (B) Forty-eight hours post transfection, MTT assay was performed to test the viability of miR-199a-3p or Mock RA-FLSs. (C,D). EdU assay of miR-199a-3p or Mock transfected RA-FLSs 48 h post transfection. Cells were stained for EdU and Hoechst (to mark nuclei) (C), and quantitated for EdU+ cell percentage (D). CCK8 assay showed that ectopic expression of miR-199a-3p significantly inhibited RA-FLS proliferation rate over 5 days. n=4 or n=6 (A,D); *P<0.05 compared with Mock.