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. 2018 Nov 14;38(6):BSR20180982. doi: 10.1042/BSR20180982

Figure 3. miR-199a-3p induced RA-FLS apoptosis.

Figure 3

(A) Caspase-3 activity was measured by a colorimetric method. (B) Western blot of cleaved caspase-3 (C-caspase-3) and cleaved PARP (C-PARP). GAPDH was the loading control. (C) Western blot of Bax and Bcl-2 protein (left) and quantitation of the Bax/Bcl-2 ratio (right). GAPDH was the loading control. (D) Summarized data of cell apoptosis (positive for Annexin V and PI) determined by flow cytometry in RA-FLS transfected with mock or miR-199a-3p. n=6; *P<0.05 compared with Mock.