Figure 5. Down-regulation of RB1 inhibited cell proliferation and induced apoptosis in RA-FLS.
(A) RB1 protein expression in RA-FLSs transfected with control (Ctrl-siRNA) or RB1-targetting siRNA (RB1-siRNA). (B) MTT assay in siRNA-transfected cells after culturing for 48 h. (C) Caspase-3 activity in siRNA-transfected cells after culturing for 48 h. (D) Western blot of C-capase-3 and C-PARP protein in siRNA-transfected RA-FLSs. GAPDH was the loading control. n=6; *P<0.05 compared with Ctrl-siRNA.