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. 2018 Nov 14;38(6):BSR20180982. doi: 10.1042/BSR20180982

Figure 6. miR-199a-3p regulated RA-FLS proliferation and apoptosis partially via suppressing RB1.

Figure 6

RA-FLS was transfected with an miR-199a-3p-expressing plasmid (miR-199a-3p) or a pSliencer 4.1-CMV puro vector (miR-control) or miR-199a-3p in combination with an RB1-overexpression plasmid (miR + pcDNA-RB1) or miR-199a-3p in combination with a negative control plasmid (miR + pcDNA-NC). (A) Western blot of RB1 protein in RA-FLSs transfected with negative control (NC) or pcDNA3-RB1. (B) Proliferation of transfected RA-FLS was measured by the MTT assay. (C) Apoptosis was measured by FITC Annexin V and PI staining followed by flow cytometry. (D) Caspase-3 activity was measured by a colorimetric method. n=6; *P<0.05. Abbreviation: NS, not significant.