Fig 2. Inhibition of PI 3 kinase blocks TGFβ induced deptor suppression and activation of mTORC1 and mTORC2.

(A–F) Serum-starved podocytes were treated with 25 micromolar Ly for none hour prior to incubation with 2 ng/ml TGFβ for 24 hours. The cell lysates were immunoblotted with the indicated antibodies. (G–L) Podocytes were transfected with the mutant p85 PI 3 kinase subunit as described in the Materials and Methods. The transfected cells were serum starved and incubated with TGFβ as described above. The cell lysates were immunoblotted with the indicated antibodies. Representative blots from four independent experiments are shown in each panel. The quantification is shown in the S3 Fig.