Fig 6. Phospho-deficient Akt2 T309A/S474A prevents TGFβ induced decrease in deptor and activation of mTORC1 and mTORC2.

(A–G) Podocytes were transfected with Akt2 T309A/S474A mutant plasmid. The transfected cells were starved and incubated with TGFβ The cell lysates were immunoblotted with indicated antibodies. Representative of 4 independent experiments is shown. The quantification is shown in the S9 Fig.