Table 1. Detection of host-specific Bacteroidales genetic markers in fecal-source samples.
Sample | No. of tested samples | No. of positive samples (% positive) | ||||||
---|---|---|---|---|---|---|---|---|
Human-specific assays | Ruminant-specific assays | Pig-specific assays | ||||||
gyrB | BacHum | HF183-TaqMan | BacR | BacCow | Pig2Bac | PF163-SYBR | ||
Raw sewage | 9 | 9 (100) |
9 (100) |
9 (100) |
1 (11) |
9 (100) |
0 (0) |
9 (100) |
Secondary-treated sewage | 9 | 8 (89) |
9 (100) |
9 (100) |
0 (0) |
1 (11) |
0 (0) |
6 (67) |
Effluent of a domestic wastewater treatment tank | 2 | 2 (100) |
2 (100) |
2 (100) |
0 (0) |
2 (100) |
0 (0) |
2 (100) |
Cattle feces | 15 | 4 (27) |
5 (33) |
15 (100) |
14 (93) |
15 (100) |
12 (80) |
15 (100) |
River water upstream of a pig farm | 9 | 8 (89) |
9 (100) |
9 (100) |
3 (33) |
7 (78) |
2 (22) |
6 (67) |
River water downstream of a pig farm | 17 | 17 (100) |
17 (100) |
17 (100) |
11 (65) |
17 (100) |
17 (100) |
15 (88) |