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. 2018 Oct 31;14(10):e1007776. doi: 10.1371/journal.pgen.1007776

Fig 2. ZHP-4 and ZHP-3 colocalize and are interdependent for their localization to the SC and crossover sites.

Fig 2

(A) α-ZHP-4 (green) and α-ZHP-3 (red) immunostaining in wild-type pachytene stage nuclei showing colocalization with the SC in early and mid-pachytene followed by co-restriction to ~6 distinct foci per nucleus in late pachytene. (B) Immunostainings of mid pachytene region nuclei of the indicated genotypes with α-ZHP-4 (green) and α-ZHP-3 (red). ZHP-4 could not be detected above background levels in zhp-3(jf61) germlines stained with DAPI (blue) and conversely no ZHP-3 could be detected in zhp-4(vv103) mutants. Scale bars 5 μm.