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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: J Neural Eng. 2018 Aug 21;15(6):066002. doi: 10.1088/1741-2552/aadbb2

Figure 9. Recording of diffusing fluorescent beads reveals minimal crosstalk between neighboring fibers.

Figure 9.

The dissociated end of a bundle of fibers was immersed in a suspension of fluorescent beads in water, while the imaging surface was recorded via a traditional fluorescence microscope. The image on the left is a standard deviation image corresponding with a 1024×1024 portion of the full sCMOS sensor, where pixel brightness corresponds with variability over the 5 second recording. It accentuates those fibers immersed in water and with diffusing beads in close proximity to the fiber tips. Traces from a selection of fibers (circled) were extracted to show intensity over the recording. Fiber intensity varies as beads diffuse around the tips of the fibers, showing a high signal-to-noise ratio and minimal crosstalk between neighboring fibers.