REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Normal goat serum	JacksonImmunoresearch	Cat#005–000-121
rabbit anti-FLAG	MilliporeSigma	Cat#F7425; RRID: AB_439687
rat anti-HA	Roche	Cat#3F10; RRID: AB_2314622
rat anti-WAKE-Ab2	Wu Lab	N/A
rat anti-GFP	Santa Cruz	Cat#101536; RRID: AB_1124404
Rabbit anti-GFP	Thermo Fisher	Cat#A-11122; RRID: AB_221569
goat anti-rat HRP	Thermo Fisher	Cat#31470; RRID: AB_228356
mouse anti-MYC	Developmental Studies Hybridoma Bank	Cat#9E10; RRID: AB_2266850
Mouse anti-BRP	Developmental Studies Hybridoma Bank	Cat#nc82; RRID: AB_2314866
Goat anti-rat IgG, Alexa488	Thermo Fisher	Cat#A-11006; RRID: AB_2534074
Goat anti-mouse IgG, Alexa488	Thermo Fisher	Cat#A32723; RRID: AB_2633275
Goat anti-rabbit IgG, Alexa488	Thermo Fisher	Cat#A27034; RRID: AB_2536097
Goat anti-mouse IgG, Alexa568	Thermo Fisher	Cat#A-11004; RRID: AB_141371
Bacterial Strains
Top10	Thermo Fisher	Cat#C404003
Chemicals, Peptides, and Recombinant Proteins
Dulbecco’s Modified Eagle Media	Thermo Fisher	Cat#11965–092
L-glutamine	Thermo Fisher	Cat#25030081
Fetal bovine serum	Thermo Fisher	Cat#16140071
Penicillin-Streptomycin	Thermo Fisher	Cat#15140122
Effectene Transfection Reagent	Qiagen	Cat#301425
0.5x RIPA Buffer	MilliporeSigma	Cat#R0278
Protein G-coated magnetic	Thermo Fisher	Cat#1003D
Dynabeads
Pierce ECL Western Blotting Substrate	MilliporeSigma	Cat#32016
DAPI	MilliporeSigma	Cat#10236276001
Paraformaldehyde, EM grade	Polysciences, Inc.	Cat#00380–250
all-trans-retinal	MilliporeSigma	Cat#R2500
Nutri-Fly Instant food flakes	Genesee Scientific	Cat#66–117
Neurobiotin	Vector Labs	Cat#SP-1120
Picrotoxin	MilliporeSigma	Cat#P1675
Mecamylamine HCl	Tocris Bioscience	Cat#2843
Escin	Santa Cruz	Cat#SC-221596
Collagenase	MilliporeSigma	Cat#C5138
Protease XIV	MilliporeSigma	Cat#P5147
Dispase	MilliporeSigma	Cat#04942078001
Streptavidin, Alexa488-conjugate	Thermo Fisher	Cat#S11223
Streptavidin, Alexa568-conjugated	Thermo Fisher	Cat#S11226
4-Aminopyridine	MilliporeSigma	Cat#A78403
Tetrodotoxin	Tocris Bioscience	Cat#1078
Cadmium chloride	MilliporeSigma	Cat#655198
Experimental Models: Cell Lines
HEK293T	American Type Culture Collection	Cat#CRL-3216; RRID: CVCL_0063
Experimental Models: Organisms/Strains
wake-GAL4	Wu Lab	N/A
UAS-CD8::GFP	Bloomington Drosophila Stock Center	BDSC: 5137
wakeD2	Wu Lab	N/A
Clkjrk	Bloomington Drosophila Stock Center	BDSC: 24515
PDF-GAL4	Bloomington Drosophila Stock Center	BDSC: 6900
R18H11-GAL4	Bloomington Drosophila Stock Center	BDSC: 48832
R18H11-LexA	Bloomington Drosophila Stock Center	BDSC: 52535
Clk4.1m-GAL4	Bloomington Drosophila Stock Center	BDSC: 36316
20xUAS-IVS-GCaMP6s	Bloomington Drosophila Stock Center	BDSC: 42749
10xUAS-IVS-myr::tdTomato	Bloomington Drosophila Stock Center	BDSC: 32222
13xLexAop2_IVS-CsChrimson.mVenus	Bloomington Drosophila Stock Center	BDSC: 55139
20xUAS-IVS-CsChrimson.mVenus	Bloomington Drosophila Stock Center	BDSC: 55135
CCAP-GAL4	Bloomington Drosophila Stock Center	BDSC: 25686
UAS-slob-HA	FlyORF	FlyORF: F003557
UAS-slob RNAi	Vienna Drosophila Research Center	VDRC: 100987
UAS-slob RNAi #2	Vienna Drosophila Research Center	VDRC: 30674
UAS-NaKβ RNAi	Vienna Drosophila Research Center	BDRC: 105863
UAS-NaKβ RNAi #2	Vienna Drosophila Research Center	VDRC: 48300
UAS-dsNR1 RNAi	C.-L. Wu	N/A
UAS-dsNR2 RNAi	C.-L. Wu	N/A
dilp2-GAL4	A. Sehgal	N/A
dilp2-mCherry	A. Sehgal	N/A
UAS-wake	Wu Lab	N/A
Recombinant DNA
pUAS-NaKβ-myc	Wu Lab	N/A
pUAST-6xmyc	Wu Lab	N/A
pUAST-WAKE-T-Flag	Wu Lab	N/A
pCMV-Slob65-HA	I. Levitan	N/A
pCMV-actin-GAL4	Wu Lab	N/A
pCMV-eGFP	Wu Lab	N/A
Oligonucleotides
GGCGCGGCCGCATGTCGG ACGATGATAAAAATC	MilliporeSigma	N/A
GCCCTCGAGTAATGAGTA GTCGGTTCGGAGTAATTTTTC	MilliporeSigma	N/A
Software and Algorithms
MATLAB R2017b	Mathworks	RRID: SCR_001622
Fiji (ImageJ)	NIH	RRID: SCR_002285
pClamp 10.3	Molecular Devices	RRID: SCR_011323
Prism 6	GraphPad	SCR_002798
Other
Dental wax	GC Corporation	Cat#27B2X00008000016
Vectashield	Vector Labs	Cat#H-1000
fiber optic cannula	Thorlabs	Cat#CFML21L10
Arduino Uno board	Arduino	Cat#A000066
collimated LED light source (625nm)	Thorlabs	Cat#M625F2
quartz glass with filament (OD/ID: 1.2/0.6mm)	Sutter Instruments	Cat#QF120–60-7.5
Borosilicate glass (OD/ID: 1.2/0.68mm)	A-M Systems	Cat#627500
syringe filter with 0.02μm pore size, Anotop10	Thermo Fisher	Cat#09–926-3