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. Author manuscript; available in PMC: 2019 Nov 15.
Published in final edited form as: Cancer Res. 2018 Sep 25;78(22):6473–6485. doi: 10.1158/0008-5472.CAN-18-1642

Figure 3: Semaphorin 7a promotes tumor-associated macrophages and lymphangiogenesis.

Figure 3:

A) In an in vivo matrigel plug assay, 66cl4 tumor cells were mixed with macrophages isolated from nulliparous or recently weaned mice (at involution day 6). The resulting plugs were harvested and F4/80/LYVE-1 co-staining quantitated. B) Growth of 66cl4 cells engineered to overexpress SEMA7A compared to empty vector controls injected into nulliparous and involution day 1 mouse mammary tissues. C) SEMA7A expression by IHC in tumors from B. D) CD45+CD31-F4/80+PDPN+ cells in tumors from B. E) %F4/80+ cells and F) # LYVE-1+ vessel density in tumors from B. G) F4/80+PDPN+ cells in E0771 SEMA7A tumors in nulliparous mice. H) LYVE-1+ density in tumors from G with the addition of tumors from SEMA7A−/− hosts injected with E0771 cells. Unpaired t-test: *p<0.05, **p<0.01, ***p<0.001.