Table 1. Antibodies used for Western blotting.
Primary and secondary antibodies used for Western blotting are listed by manufacturer, catalogue number and lot number, molecular weight, and species of origin; antibody specificity to human (H), mouse (M), and rat (R) is also indicated.
| Cell Signaling Technology |
Cat # |
Lot # |
Molecular Mass (kDa) |
Species of Origin |
Specificity |
|---|---|---|---|---|---|
| O-GlcNAcylation | 9875 | 4 | Mouse | H, M, R | |
| PERK-P | 3179 | 19 | 170 | Rabbit | M, R |
| PERK | 3192 | 10 | 140 | Rabbit | H, M, R |
| eIF2a-P | 3398 | 6 | 38 | Rabbit | H,M,R |
| eIF2a | 9722 | 5 | 38 | Rabbit | H, M, R |
| Protein Tech |
Cat # |
Lot # |
Molecular Mass (kDa) |
Species of Origin |
Specificity |
| GFAT1 |
14132–1-AP |
5163 |
77 |
Rabbit |
H, M, R |
| Abcam |
Cat # |
Lot # |
Molecular Mass(kDa) |
Species of Origin |
Specificity |
| GFAT2 |
ab190966 |
GR243495–2 |
77 |
Rabbit |
H, M, R |
| Origene |
Cat # |
Lot # |
Molecular Mass (kDa) |
Species of Origin |
Specificity |
| DDK (FLAG tag) |
TA5011–100 |
A043 |
Mouse |
M |
|
| Santa Cruz |
Cat # |
Lot # |
Molecular Mass (kDa) |
Species of Origin |
Specificity |
| GAPDH |
sc-32233 |
K0315 |
37 |
Mouse |
H, M, R |
| α-tubulin |
sc-32293 |
C0112 |
55 |
Mouse |
H, M, R |
| Bethyl Laboratories |
Cat # |
Lot # |
Molecular Mass (kDa) |
Species of Origin |
Specificity |
| Secondary antibody |
A120–101P | 40 | Goat | R | |
| Secondary antibody |
A90–116P |
38 |
Goat |
M |
|