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. Author manuscript; available in PMC: 2019 Nov 15.
Published in final edited form as: Cell Chem Biol. 2018 Sep 27;25(11):1419–1427.e4. doi: 10.1016/j.chembiol.2018.09.004

Figure 1. Cataloguing endogenously synthesized metabolites using deep labeling.

Figure 1

(A) Schematic of the deep labeling experiment strategy. (B) 13C enrichment of 1,319 LC-HRMS peaks with a unique predicted formula, in extract of cells grown in 12C medium (blue) or 13C medium (black). Individual enrichment values for three replicates are shown. (C-E) MIDs of pyruvate, acetyl-CoA and a novel pentose acid (ribonate; see text) in cells grown in 12C medium (blue) or 13C medium (black). (F-H) MIDs of pentose acid (ribonate), gulonate and ribose-5-phosphate/ribulose-5-phosphate in cells cultured with 1-13C1-glucose. Ribose-5-phosphate and ribulose-5-phosphate could not be separated by chromatography. Error bars denote standard deviation. MI fractions not shown were negligible. See also Figure S1 and Figure S2.