Key Resources Table.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Methanol (JT Baker), HPLC grade | VWR | Cat#BAKR8402.250 0 |
| Ammonium Bicarbonate | Honeywell | Cat#40867-50G |
| LCMS Grade Water | Optima | Cat#W64 |
| LCMS Grade Methanol | Honeywell | Cat#BJLC230-4 |
| LCMS Grade Acetonitrile | Honeywell | Cat#BJLC015-4 |
| 8M Urea | Sigma-Aldrich | Cat#U4883 |
| RPMI 1640 vitamins solution 100x | Sigma-Aldrich | Cat#R7256 |
| Trypsin-EDTA (0.05%), phenol red | Gibco | Cat#25300062 |
| PBS, pH 7.4 | Gibco | Cat#10010056 |
| Fetal bovine serum, qualified, heat inactivated US origin | Gibco | Cat#16140071 |
| Penicillin-Streptomycin (10,000 U/mL) | Gibco | Cat#15140122 |
| Phenol red solution | Sigma-Aldrich | Cat#P0290 |
| D-Glucose (dextrose) | Sigma-Aldrich | Cat#D9434 |
| Glycine | Sigma-Aldrich | Cat#50046 |
| L-Arginine | Sigma-Aldrich | Cat#A8094 |
| L-Asparagine | Sigma-Aldrich | Cat#A4159 |
| L-Aspartic acid | Sigma-Aldrich | Cat#A7219 |
| L-Cysteine | Sigma-Aldrich | Cat#C7352 |
| L-Glutamic acid | Sigma-Aldrich | Cat#G8415 |
| L-Glutamine | Sigma-Aldrich | Cat#G8540 |
| L-Histidine | Sigma-Aldrich | Cat#H6034 |
| trans-4-Hydroxy-L-proline | Sigma-Aldrich | Cat#H54409 |
| L-Isoleucine | Sigma-Aldrich | Cat#I7403 |
| L-Leucine | Sigma-Aldrich | Cat#L8912 |
| L-Lysine monohydrochloride | Sigma-Aldrich | Cat#L8662 |
| L-Methionine | Sigma-Aldrich | Cat#M3508 |
| L-Phenylalanine | Sigma-Aldrich | Cat#P5482 |
| L-Proline | Sigma-Aldrich | Cat#P5607 |
| L-Serine | Sigma-Aldrich | Cat#S4311 |
| L-Threonine | Sigma-Aldrich | Cat#T8441 |
| L-Tryptophan | Sigma-Aldrich | Cat#T8941 |
| L-Tyrosine | Sigma-Aldrich | Cat#T8566 |
| L-Valine | Sigma-Aldrich | Cat#V0513 |
| Calcium nitrate tetrahydrate | Sigma-Aldrich | Cat#C2786 |
| Sodium phosphate dibasic | Sigma-Aldrich | Cat#S0876 |
| Magnesium sulfate | Sigma-Aldrich | Cat#M2643 |
| Potassium chloride | Sigma-Aldrich | Cat#P5405 |
| Sodium bicarbonate | Sigma-Aldrich | Cat#S5761 |
| Sodium chloride | Sigma-Aldrich | Cat#S7653 |
| L-Glutathione reduced | Sigma-Aldrich | Cat#G6013 |
| 4-Methyl-2-oxovaleric Acid Sodium Salt (4mop) | Toronto Research Chemicals | Cat#M326425 |
| α-Keto Isovaleric Acid Sodium salt (3mob) | Toronto Research Chemicals | Cat#K193500 |
| 3-Methyl-2-oxovaleric Acid Sodium Salt (3mop) | Toronto Research Chemicals | Cat#M326420 |
| D-Glucose (U-13C6, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-1396-0 |
| Glycine (U-13C2, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-1017-0 |
| L-Arginine (U-13C6, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-2265-H |
| L-Asparagine (U-13C4, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-8699-H |
| L-Aspartic acid (U-13C4, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-1801-H |
| L-Cysteine (U-13C3, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-4320-H |
| L-Glutamic acid (U-13C5, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-1800-H |
| L-Glutamine (U-13C6, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-1822-H |
| L-Histidine (U-13C6, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-2264-0 |
| L-Isoleucine (U-13C6, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-2248-H |
| L-Leucine (U-13C6, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-2262-H |
| L-Lysine hydrochloride (U-13C6, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-2247-H |
| L-Methionine (U-13C5, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-893-H |
| L-Phenylalanine (U-13C9, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-2250-H |
| L-Proline (U-13C5, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-2260-H |
| L-Serine (U-13C3, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-1574-H |
| L-Threonine (U-13C4, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-2261-0 |
| L-Tryptophan (U-13C11,99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-4290-H |
| L-Tyrosine (U-13C9, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-2263-H |
| L-Valine (U-13C5, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-2249-H |
| D-Glucose (2-13C, 99%) | Cambridge Isotope Laboratories, Inc | Cat#CLM-746-PK |
| D-Glucose (1-13C) | Omicron Biochem. Inc. | Cat#GLC-018 |
| Critical Commercial Assays | ||
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| HCT116 | ATCC | Cat#ATCC® CCL-247™ |
| MCF7 | ATCC | Cat#ATCC® HTB-22™ |
| HeLa | ECACC | Cat#93021013 |
| Experimental Models: Organisms/Strains | ||
| Oligonucleotides | ||
| Recombinant DNA | ||
| Software and Algorithms | ||
| mzAccess (http://www.mzaccess.org/) | (Lyutvinskiy et al. 2017) |
N/A |
| Mathematica v10 | Wolfram Research | N/A |
| GraphPad Prism version 7 software | GraphPad Software | N/A |
| Other | ||
| SnakeSkin™ Dialysis Tubing, 3.5K MWCO, 35 mm | Thermo Fisher Scientific |
Cat#88244 |
| Cell scraper with two-position blade, scraper length: 25 cm | Sarstedt | Cat#83.1830 |
| Cell culture dish 10 cm | Sarstedt | Cat#83.1830 |
| Cell culture plates, 6-well | Sarstedt | Cat#83.3920 |