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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: J Neurooncol. 2018 Aug 21;140(2):209–224. doi: 10.1007/s11060-018-2950-1

Figure 5. RA and UAB30 decreased MB PDX cell invasion and migration.

Figure 5.

a For cell migration, cells (1 × 106) were plated in a 6 well culture plates and treated with RA or UAB30 (0 μM, 10 μM, 30 μM) for 24 hours. Treated cells (1.5 × 105) were plated in Transwell® culture plates in the upper well with neurobasal media; 10% fetal bovine serum was added to the lower chamber as a chemoattractant. The cells were allowed to migrate for 24 hours, then were fixed, stained and counted and migration reported as fold change ± SEM. Migration was significantly inhibited in all 3 PDXs with 10 μM RA and UAB30. Representative photomicrographs of the 10 μM treated migration plates are provided below the migration graphs. b Invasion was assessed in a similar fashion. Treated cells were plated in Transwell® culture plates with Matrigel™ coating the top side of the insert. Cells were allowed to invade for 24 hours, and then fixed, stained and counted. Invasion was reported as fold change ± SEM. Invasion was significantly decreased in all 3 MB PDXs beginning at 10 μM RA or UAB30. Representative photomicrographs of the 10 μM treated invasion plates are provided below the graphs.