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Radiation + α-OX40 upregulated CD4+ and CD8+ tumor-infiltrating T-cells in primary (irradiated) and second (non-irradiated) tumors. In the abscopal model, primary tumors A, B, C, (injected in the right hind leg and later irradiated) and secondary tumors D, E, F, (injected in the left leg and not irradiated) were harvested, processed, and analyzed by flow cytometry on day 29 for CD4+ and CD8+ T-cells after gating on CD45+ cells. *P ≤ 0.05 is considered significant.
