Figure 3. Effect of catalase overexpression on cyto-HyPer.
(A) Western analysis of cytosolic catalase in islets and mitochondrial catalase in INS-1 cells Glucose-induced increase in cyto-HyPer signal is suppressed in response to (B) overexpression of cytosolic catalase in rat islets (n = 4) and (C) overexpression of mitochondrial catalase in INS-1 cells (n = 4), demonstrating specificity of HyPer for H2O2.