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. 2018 Nov 16;8:16931. doi: 10.1038/s41598-018-35184-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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VNN2, GINS4, CTNNBL1, and SULF2 are up regulated by cAMP in human monocytes. Panel (A–D) Human monocytes were isolated as described in the Methods. These cells were exposed for 6 h to 10 nM ET (10 nM EF and 10 nM PA) or 500 μM 6MB-cAMP. RT-qPCR was then used to quantify transcript levels of VNN2, GINS4, CTNNBL1, and SULF2. Bar graphs are representative of 3 independent experiments with monocytes derived from different donors. Error bars indicate mean ± S.D. Asterisks indicate significant different than control cells. *p < 0.05.