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. 2018 Nov 16;8:16931. doi: 10.1038/s41598-018-35184-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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C/EBP β and CREB bind the promoter of TLE genes, and TLE genes are induced by cAMP in human monocytes. Panel (A–C) C/EBP β and CREB ChIP-seq analysis were performed on human PBMC pooled from 3 donors. Displayed here are the UCSC genome browser views of C/EBP β and CREB binding the TLE1, TLE3, and TLE4 genes. Panel (D–F) Human monocytes were isolated as described in the Methods. These cells were exposed for 6 h to one of the following or a combination of the following: 10 nM ET (10 nM EF and 10 nM PA), 1 mM 6MB-cAMP, or 100 ng/ml LPS. RT-qPCR was then used to quantify transcript levels of TLE1, TLE3, and TLE4. Bar0 graphs are representative of 3 independent experiments with monocytes derived from different donors. Error bars indicate mean ± S.D. Asterisks indicate significant different than control cells. *p < 0.05.