Fig. 1.

CRIPSPR-Cas9-based knock out of the CYP6AE cluster of H. armigera. a Positions of the two sgRNAs (sgRNA14 and sgRNA12) and the four primer pairs for allele-specific PCR detection. b Target sequences of the two sgRNAs (blue), the PAM sequences (red), and a representative chromatogram of direct sequencing of PCR products of individuals from the SCD-d6AE strain with the primer pair 14F/12R. The cutting sites by the Cas9 protein are indicated with red triangles. c Genotyping of individual H. armigera for deletion of the CYP6AE cluster according to banding patterns of the PCR products amplified with a set of four primer pairs