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. 2018 Nov 16;8:16957. doi: 10.1038/s41598-018-35150-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The DUX4 C-terminal activation domain is not necessary for inhibition of myogenesis. (A) RT-qPCR for MYOD1, MYF5, DUX4 target genes ZSCAN4, MBD3L2 in cells induced with 200 ng/mL dox for 2 and 14 hours. (B) Immunostaining for MHC (red) after 2 days of myogenic differentiation. Nuclei are visualized with DAPI (blue) staining. DUX4 deletion constructs were induced with 200 ng/mL dox. (C) RT-qPCR for myogenic genes in cells represented in (B). Data are presented as mean ± SEM; **p < 0.01, ****p < 0.0001, by one-way ANOVA with Tukey’s post hoc test. Results presented as fold difference compared to control uninduced cells (n = 4). (D) Immunostaining for DUX4C (red), MHC (green) and DAPI (nuclei, blue) in LHCN-M2-iDUX4C cells after 2 days of myogenic differentiation and induction with 200 ng/mL dox. Note that DUX4C is expressed (red) in cells that were not able to terminally differentiate. Scale bar 100 µm.