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. 2018 Nov 16;8:16961. doi: 10.1038/s41598-018-35377-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genomic comparison of genes coding for T6SS system. (A) Distribution of the T6SS components in Acinetobacter. Each component of the T6SS was mapped on the 96 sampled genomes, representatives of the diversity of the genus. Genes were searched by means of BLASTP, using a maximum e-value of 1^e-5, a minimum identity value of 50%, and query coverage of 75% as threshold. The genes of A. baumannii AYE were used as query in all cases, as defined in Weber et al. 2013. Orange boxes show presence and gray boxes show absence of T6SS genes. The heatmap was generated using GraphPad Prism version 7 (B) Bacterial Killing assay using E. coli TOP10-AK (Prey) and Ab33405 (Predator). Representative LB-AK agar plate showed differences in survival of bacterial colonies. The experiment was performed two times.