Fig. 1.

Responses to Candida spp. in neonatal immune cells. a Phagocytosis of Candida in monocytes (boxes and whiskers), including a representative flow microscopy diagram (white bar = ∼10 µm). Data pooled from multiple experiments over 14 months (9 to 17 subjects per age group; see Supplemental Data for clinical information on preterm subjects); b IL-1β and c IL-6 response (blood mononuclear cells) to C. albicans or C. parapsilosis (24 h stimulation; 10 to 18 subjects per age group; boxes and whiskers); (d) IL-1β (24 h; 11 to 21 subjects per age group; boxes and whiskers) and e representative gating for pro-IL-1β (5 h LPS stimulation), gated on CD14-expressing cells (black = fluorescent-minus one control; orange = unstimulated; blue = LPS; representative preterm sample is from a 26 weeks’ infant); f pro-IL-1β (5 h) or g IL-6 (24 h) in response to LPS, zymosan or curdlan (mononuclear cells; 11 to 21 subjects per age group; boxes and whiskers); for b and c, data was pooled from multiple experiments assayed in four ELISA batches with similar distribution of samples per age group