Table 2.
Primary and secondary antibodies used in Western blot analysis.
| Protein | Dilution of primary and secondary antibodies | Company |
|---|---|---|
| NOTCH1 | Rabbit anti-NOTCH1 C20R (1:1000) | Santa Cruz Biotechnology |
| Active NICD1 | Rabbit anti-Cleaved NOTCH1 (Val 1744) (1:000) | Cell signaling |
| NOTCH2 | Goat anti-NOTCH2 M20 (1:500) | Santa Cruz Biotechnology |
| NOTCH3 | Rabbit anti-NOTCH3 (1:1000) | Abcam |
| NOTCH4 | Rabbit anti-NOTCH4 (1:1000) | Upstate Millipore |
| HA | Mouse anti-HA 16B12 (1:5000) | Covance |
| DLK1 | Rabbit anti-DLK1 (1:1000) | Nueda et al.49 |
| DLK2 | Rabbit anti-DLK2 (1:500) | Proteintech |
| α-tubulin | Mouse anti-alpha-Tubulin (1:5000) | Sigma |