Table 3.

Oligonucleotides used in qRT-PCR assays to quantify the expression of the genes indicated.

Gene	Oligonucleotide sequences
Ucp1	mUcp-1up: 5′-CAATTGTACAGAGCTGGTAAC-3′
	mUcp1-down: 5′-TGTTTTTACCACATCCACGT-3′
Gyk	mGyk-up: 5′-ATCTATGGCCTAATGAAAGC-3´
	mGyk-down: 5′-GAAAATACACACTTATGGCCC-3′
Pgc1a	mPgc1-a-up: 5′-TCCTCTTCAAGATCCTGTTAC-3′
	mPgc1-a-down: 5′-CACATACAAGGGAGAATTGC-3′
Prdm16	mPrdm16-up: 5′-ATCTACAGGGTAGAAAAGCG-3′
	mPrdm16-down: 5′-TCTCCGTCATGGTTTCTATG-3′
Cidea	mCidea-up: 5′-GTGTTAAGGAATCTGCTGAG-3′
	mCidea-down: 5′-CTATAACAGAGAGCAGGGTC-3
Sirt1	mSirt1-up: 5′-AAACAGTGAGAAAATGCTGG-3′
	mSirt1-down: 5′-′GGTATTGATTACCCTCAAGC-3