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. 2018 Nov 16;8:16951. doi: 10.1038/s41598-018-34855-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Safranal arrests HepG2 cells at G2/M and S Phase and affects cell cycle regulators. (a) Cell cycle progression of HepG2 cells after treatment with safranal at a dose of 500 µM over a period of 48 h; and quantitative distribution of HepG2 cells in different phases of the cell cycle at different time intervals. Statistical analysis was carried out by student’s t-test using GraphPad Prism software and p < 0.05 was considered as statistically significant. *p < 0.05 and ***p < 0.01 (b). Western blot analysis of cell cycle regulatory proteins in HepG2 cells post treatment with safranal at a dose of 500 µM. Each band intensity was quantified using ImageJ, normalized relative to their respective loading control bands. Values are expressed as ratio of untreated control. Western blot images (b) have been cropped for clarity with full blots presented in Supplementary Fig. 1. (c) Best docked poses of safranal within the human CDC25B binding site.