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. 2018 Nov 16;8:16967. doi: 10.1038/s41598-018-35395-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ARL2-Q70L expression affects ciliary stability and ciliary length in RPE1-hTERT cell lines. (A) Immunofluorescence analysis of RPE1-hTERT cell sublines with HA-tagged ARL2-WT or ARL2-Q70L at 48 hours of FBS(−) media incubation without (Dox(−)) or with (Dox(+)) doxycycline added at 24 hours of FBS(−) media incubation. Demonstrating immunoreactivity for DAPI (blue), HA (green), Acetyl α-tubulin (red), and γ-tubulin (magenta) (Scale Bar = 5 μm; Scale Bar (zoomed) = 3 μm). (B) Bar graphs illustrating the fraction ciliated (Ciliation) (Top) and average ciliary length (Bottom) normalized to Dox(−) control using at least 100 cells and 80 cilia per replicate. n = 3, *p < 0.05.