Figure 2.

ZFP91 enhances the expression of non‐canonical caspase‐8 inflammasome components in LPS‐stimulated THP‐1 cells and BMDMs. (A, B) PMA‐differentiated THP‐1 cells were transduced with lentiviruses containing ZFP91 gene using multiplicities of infection (MOI) of 10, 50 and 100 or lentiviruses carrying ZFP91 siRNA using MOI of 50 and 100 for 24 h and then stimulated with 1 μg·mL⁻¹ LPS for 6 h. The protein levels of NLRP3, ASC, cleaved caspase‐8 and cleaved caspase‐1 were measured by Western blot analysis. (C, D) PMA‐differentiated THP‐1 cells were transduced with lentiviruses containing ZFP91 gene using MOI of 10, 50 and 100 or lentiviruses carrying ZFP91 siRNA using MOI of 50 and 100 for 24 h and then stimulated with 1 μg·mL⁻¹ LPS for 6 h. The mRNA levels of NLRP3, ASC, caspase‐8 and caspase‐1 were measured by RT‐PCR. (E) BMDMs from wild type (WT) or ZFP91 knockout (ZFP91^−/−) C57BL/6 mice stimulated with 1 μg·mL⁻¹ LPS for 6 h. The protein levels of NLRP3, ASC, cleaved caspase‐8 and cleaved caspase‐1 were measured by Western blot analysis. (F) BMDMs from WT or ZFP91 knockout (ZFP91^−/−) C57BL/6 mice stimulated with 1 μg·mL⁻¹ LPS alone or with caspase‐1 inhibitor for 2 h. The protein levels of NLRP3, ASC, cleaved caspase‐8 and cleaved caspase‐1 were measured by Western blot analysis.